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Product Detail
Magigen LAMP Amplificatoin Kit | LAMP Amplification Mix Kit 50T
Magigen LAMP Amplificatoin Kit | LAMP Amplification Mix Kit 50T
Name: LAMP Amplification Mix Kit
Catalog: F001S
Unit Size: 50T
Price:
$203.00
Storage Conditions: Store at -20°C
Port: Guangzhou
Quantity:
Detail
  • Product name

    Magigen MagicScript 2xLAMP Amplification Mix with dye

  • Catalog

    F001S/L

  • Intended use

    Magigen MagicScript LAMP kit is one-step solution for Loop-Mediated Isothermal Amplification(LAMP) of DNA targets. The kit contains fluorescent dye, dNTPs, metal ion indicators for LAMP reaction.

  • Size

    50T/500T

  • Storage conditions and Expiration

    Storage conditions: -20℃,avoid light.

    Expiration: 12 months.

  • Contents of the kit




    Item

    F001S

    F001L




    LAMP Amplification Mix

    625μLx1Tube

    625μLx10Tube


  • Detection principle

    LAMP, Loop-mediated isothermal amplification, uses 4 primers recognizing 6 distinct regions of target DNA fora highly specific amplification reaction. This method employs a strand-displacing DNA polymerase and a set of four specially designed primers that recognize a total of six distinct sequences on the target DNA. The method provides high amplification efficiency of specific DNA, up to 10 fold, at a constant temperature (about 60-65 °C) in 15-60 Min. it is simple, high specificity, efficiency and rapidity. The products would enable their simple, easy and selective detection.

  • Applicable instrument

    You can use a fluorescence quantitative PCR instruments, like Slan-96P,Strongmed, Shanghai, or MA-1600Q series Real-Time Quantitative Thermal Cycler.

  • Magigen LAMP kit DATA

    Ultra sensitive

    The sensitive is up to 10 copies/reaction when using the Magigen LAMP Kit to test the African Swine Fever Virus (ASFV) sample. It is stable at 100 copies/reaction.

    ultra sentive amplification kits-Magigen LAMP kits


    Fig1. From left to right are the amplification curves of 10,000, 100 and 10 copies respectively.



    Good stability

    The nucleic acid amplification results showed that the performance remained unchanged after the kit repeated freezing and thawing for 1, 2, 3, 4, 5 times .


    ultra sentive high stability amplification kits-Magigen LAMP kits


  • Operation procedure

    1. DNA extraction.

    You can use commercial DNA extraction kits to do DNA extraction. Spin column and magnetic bead method are recommended.

    2. Methods

    2.1 Take the components out, melt slowly on ice or at 4℃, mix well, centrifuge briefly, and set aside.

    2.2 Make reaction mix in a centrifuge tube with the components in the list below.

    ComponentRecommended volume(μL)
    2×LAMP   Amplification Mix12.5
    F3/B3   Primers(10μM)2
    FIP/BIP Primers(10μM)4
    LF/LB(10μM)(opt)2
    Template 2
    RNase-free   WaterUp   to 25



    Vortex immediately after preparation and spin down gently, mix well, centrifuge briefly, put it into the instrument immediately to react.


  • 2.3 put the tube into the fluorescence quantitative PCR instruments with FAM channel, set the temperature to 60-65℃ (65℃ is recommended), reaction time30-60 min. The nonspecific amplification will increase significantly when reaction time beyond 50 min. Collect the fluorescent data every 60 second.

  • Note:

    a. Generally, the reaction time is set to 45 min. The reaction time could be prolonged to 60 min if Loop primers are not used.

    b.   The primer combination and volume can be optimized and adjusted according to the actual situation.

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