Taq DNA Polymerase

Magigen MagicScript Antibody Taq DNA Polymerase | Antibody Taq Polymerase

● Product Name: Magigen ABM Hotstart Taq DNA Polymerase 1.0   

● Catalog#: A007L

● Size: 5000U

● Contents

*1. Contains 3 mM Mg²⁺

● Advantages and Features

Magigen ABM Hotstart Taq DNA Polymerase 1.0 is the antibody-mediated hot start Taq Polymerase that enhances the specificity and sensitivity of PCR, which can be fully activated after heating at 95 ℃ for 30sec.   

The ABM Taq uses gene specific primers (GSP) with the optimized reaction system for a probe-based qPCR reaction.

Meanwhile, ABM HotStart Taq DNA polymerase 1.0 has 5 '-3' DNA polymerase activity and 5 '-3' exonuclease activity specific for double stranded DNA; No 3 '-5' exonuclease activity.

This product can also be used for ordinary PCR amplification, and the 3 'end of the obtained product is attached with a base "A", which can be directly used for TA cloning.

● Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 74°C with the activated salmon sperm DNA as the template-primer.

● Storage Conditions and Shelf Life

Sealed and stored at -20 ℃,   12 months.

➢ What is Taq Polymerase used for?

◆for ordinary PCR reaction

◆for probe-based qPCR reaction

◆for TA clone

● Recommended Magigen Taq Reaction System

Composition                                                   Vol

2×Reaction Buffer 4                                       12.5 μL

ABM Hotstart Taq 1.0 DNA Polymerase          0.25~1μL

Foward Primer(10 μM)                                    0.5 μL

Reverse Primer(10 μM)                                   0.5 μL

Probe（10 μM）²                                           0.25 μL

template                                                        1 pg~100 ng

ddH₂O                                                            Up to 25 μL

*2. It needs to be added when performing fluorescent qPCR.

● Recommended amplification procedure

◆Two steps method

Cycle step                   Temperature       Time          Number of cycles

Pre denaturation           95 ° C            30s-4min3         1

Denaturation                 95 ℃             15sec                45

Annealing/extension     60 ℃            30sec Fluorescence collected   

◆ Three steps method

Cycle step                   Temperature       Time          Number of cycles

Pre denaturation           95 ° C            30s-4min³         1

Denaturation                 95 ℃             15sec                28~45

Annealing                      60 ℃ ⁴          30sec  

Extension                       72℃              60s/kb

Extension                      72℃             7min                  1

*3. The denaturation time can be increased to 4min for complex template.

*4. The annealing temperature should be adjusted according to the primer Tm value.

● Related Taq Polymerase Price (USD)

Note:

Magigen Hotstart Taq DNA Polymerase is a qualified enzyme with good price.

The hotstart taq polymerase price will be subject to change without prior notice due to currency fluctuation.

All Pictures shown are for illustration purpose only and shall not form part of any offer, contract.