Nucleic Acid Test
Reverse Transcription Kit
● Product Name: Magigen Reverse Transcriptase II - RT enzyme For RT-PCR Test
● Catalog# : A001M
● Size: 500µl , 200U/ µl
● Reverse Transcriptase Price: 283USD
● Contents
Composition Vol
5× Reaction Buffer 1 1 ml×3
thermostable reverse transcriptase II 500 µl×1
100 mM DTT 500 µl×2
ddH2O 1.5 ml×2
● Magigen RT enzyme Features
Magicscript Reverse Transcriptase, RTase II, is a recombinant DNA polymerase. It is a product of the pol gene of Moloney Murine Leukemia Virus （M MLV）. Based on the reduction of RNase H activity , genetically engineered, Magicscript Reverse Transcriptase II works perfect over a wider temperature range with higher thermal stability and longer half-life.
● Unit Definition
One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-insoluble material in 10 minutes at 37°C using poly(rA)•oligo(dT) as template/primer.
● Storage Conditions and Expiration
Sealed at-20℃, valid for 12 months.
● Product Performances
Comparing Magicscript RT-II with same products from both domestic and oversea suppliers under same reacting conditions of 42℃ and 55℃, the results present reliability and stability, especially the superiority in higher temperature zone.
● Magicscript Reverse Transcriptase II Advantages
1. A wider thermal resistance range of 37°C to 65°C compared with other M-MLV product, and high activity. The residual activity retains at least 70% after 30 min in the high temperature range of 55°C to 65°C.
2. Work with kinds of reaction buffers, compatible with one-step reaction systems such as RT-PCR\RT-RDA\RT-Lamp\RT-HDA.
3. Show a perfect linear relationship between the different RNA concentration and the cDNA yield.
● How to use thermostable Reverse Transcriptase II
Example: first strand cDNA synthesis
Reaction System Vol ( µl)
Thermostable RTase II 1
5× Reaction Buffer 1 4
100 mM DTT 2
RNase Inhibitor（20-40 U/µl） 1
dNTP Mix ( 25 mM each) 1
primer 10μM-250μM
template 1pg-100ng
ddH2O Up to 20-25
● Reaction steps
1. gently mix the reaction system;
2. recommended reaction procedure:
Temperature Time
25℃1 2min
45℃ 30min
85℃ 2min
*1. This step is only required when using random hexmers;
● RTase II Kit Application
The obtained cDNA can be directly used for 2nd strand cDNA synthesis or PCR amplification.
● Heat Inactivation
80°C for 20 minutes
● Note
1. The optimal temperature range of magicscript thermostable reverse transcriptase II is 37-45 ℃, Its RNaseH activity was one-third that of wild-type MLV. When the temperature is higher than 45 ℃ (lower than 65 ℃), the residual activity will decrease correspondingly with the increase of temperature, but at least 70% of the activity can still be maintained.
2. When using this product for one-step RT-PCR experiments, it is recommended to add the following components to the complete PCR system (including templates, specific primers and other components) that researchers have:
Reaction System Vol ( µl)
Thermostable RTase II 1
RNase inhibitor（20-40 U/µl） 1
Final volume 20-25
Suggested reaction procedure:
45-50℃ 15 min
PCR procedure
● Notice
Magigen Reverse Transcription Kit is a high quality product with a good price.
The Reverse Transcription Kit price is subject to change without prior notice due to currency fluctuation.
The Reverse Transcription Kit price is FOB price, not include tariffs, freight, insurance and other expenses. Some products need to be delivered by dry ice. Please consult the sales manager for details before purchasing.
All Pictures shown are for illustration purpose only and shall not form part of any offer, contract.
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